I-PCR kwintlobo yesifo somzimba (indlela ye-polymeterase reaction reaction method) yindlela yokuchonga izifo ezichaphazelekayo, ezisekelwe ekuzimweni kwezinto eziphathekayo ezivela kwisigulane. Ekuqhubekeni kwesi sifundo, izinto zifakwe kwi-reactor ekhethekileyo. Njengoko isampula yokuvavanya inokusebenza: ukufihla, igazi, i-muscus. Izinto ezikhethekileyo ze-enzymatic zongezwa kwisampuli ezithathwe. Ngoncedo lwabo, ikopi ye-DNA ye-pathogen ihlanganiswe. Le mpendulo iyimvelo yechungechunge. Kule ndlela kwaye igama layo laligama.
Kusetyenziswa nini?
Ukuxilongwa kosulelo lwe-PCR yinkqubo eyinkimbinkimbi, ukuchazwa kweziphumo zazo ezijongene nazo iingcali. Le ndlela inceda ukufumanisa izifo ezininzi ezifihliweyo ezifakiwe kwi-PCR:
- papillomavirus;
- urogenital (chlamydia, trichomoniasis, ureaplasmosis ).
I-PCR yindlela ephezulu yokufumanisa usuleleko lwe-HIV.
Inkcazo
Emva kokuxilongwa kwezifo ezisebenzisayo usebenzisa indlela ye-PCR, iziphumo zophando ziphethwe. Kule meko, iifom yezinto ezimbini zisetyenzisiweyo: "isiphumo esibi" kunye "nesiphumo esihle".
Ngeniphumo emihle, oogqirha banokuthi bathe ngokuqinisekileyo ukuba kukho enye okanye enye i-agent engumzekelo kumzimba wesifundo. Isiphumo esibi sibonisa ukungabikho kokutheleleka ngokupheleleyo kumzimba womntu.
Izibonelelo ze-PCR
Le ndlela yokuxilonga inezinzuzo ezininzi, eziphambili zazo ezi:
- Ukuxilongwa ngokuthe ngqo kobukho be-pathogen emzimbeni. Ezinye iindlela zokuxilongwa zingabonisa umxholo emzimbeni weeprotheni-markers kuphela. I-PCR ibonisa ngokucacileyo ubukho emzimbeni wesifundo esithile se-pathogen.
- Umgangatho ophezulu wecandelo. Oku kubangelwe ukuba inqaku lezinto ezifundwa ngamagqirha
ummandla we-DNA chain of the pathogen uchongiwe, apho kuchongwa khona. - Ukuphakama okukhulu kwindlela. Indlela ye-PCR yenza ukuba kunokwenzeka ukuba kuchonge amaseli angatshatanga. Le propati ibaluleke kakhulu, kuba amaninzi amagciwane ayaba nelungelo elifanelekileyo kwaye liyingozi kwimpilo yabantu kuphela kwinani elikhulu. Ngombulelo kwi-PCR, isifo singasungulwa ngaphandle kokulinda umzuzwana.
- Ukukwazi ukuxilonga ngezifo ezininzi, kuthatha isampuli esisodwa sezinto eziphathekayo.