I-vitrification yama-Embryo

Olu hlobo lwenkqubo, olufana ne-vitrification ye-embrosi, yenye yeendlela zokwenza i- cryopreservation (iqhwa). Isetyenziswe xa kuyimfuneko ukuyeka i-protocol ye-IVF. Ngokuqaliswa kwale ndlela, kwakunokwenzeka ukuphucula ngokukodwa izinga lokusinda kweesibini zesini kunye nemibungu emva kwenkqubo yokuqhawula.

Kufuneka kube nini ukukhupha imibusi?

Kufuneka kuqatshelwe ukuba i-cryopreservation inokwenziwa nawaphi na amanqanaba okuphuhlisa (i-pronucleus, i-crush epbrus, i-blastocyst). Ngenxa yokuba le nkqubo ingasetyenziselwa phantse nayiphi na ixesha, xa kunokwenzeka ukuba awuphumelelanga ukufika kwe-uterus.

Ngokumalunga neenzuzo ezikhawulezayo zokubanda, phakathi kwezi zimele zibizwa ngokuba:

1. Ukwandisa amathuba okukhulelwa emva kwe-IVF kunye nokuthintela ukufa kweemibusi eziqhelekileyo, ezenza ukuba zikwazi ukuzisebenzisa emva kokufumba.
2. Ithintela imiphumo yoxinzelelo phambi kobuchule obuphezulu bokuphuhliswa kwayo.
3. Isisombululo kwingxaki apho ukuvumelanisa kwimijikelezo yokuya esikhathini kunye nommkeli akunakwenzeka.

I-cryopreservation ye-embrosiyiti yindlela yokwenza i-vitrification iyimfuneko xa:

• kuncipha kwiimeko zokungeniswa kwendawo yomzimba, ngenxa yobuninzi bezinto eziphazamisayo kwi-organism (i-polyps, umzekelo);
• i-stenosis yomsele womlomo wesibeleko (akukho catheter inokufakwa kwi-canal yomlomo).

Ukuqhwala kukuchaphazela njani umbungu?

Kwimeko yezifundo ezininzi zophando, kwafunyaniswa ukuba ukufeza le nkqubo akunakuchukumisa ukuphuhliswa kombindi. Ngoko ke, ukuba kuyimfuneko, i-biomaterial ichithwe kwi-capsule ene-nitrojeni yamanzi, ishiywe kwiqondo lokushisa lama-20-22 degrees, emva koko i-cryoprotectant isuswe kwaye i-embryo ifakwe kwisithuba esikhethekileyo. Emva kokuvavanya imeko yembungu, qhubela phambili kwinkqubo yokutyala.