Ukugcina imbewu

Le ndlela yokugcina isistim (i-ejaculate), njenge-cryopreservation, isanda kakhulu kwi-cryomedicine. Kuquka ukongezwa kwendawo ekhethekileyo ukuya kwi-seminal fluid, kunye nokuyikhupha ngokusetyenziswa kwamanzi omhlaba we-nitrogen. Cinga ngale ndlela yokulondoloza iiseli zentsholongwane yendoda ngokubanzi kwaye ikuxelele ukuba yiyiphi i-sperm efanelekile yokuhlengahlengiswa kunye nokugcinwa okuqhubekayo.

I-cryopreservation ikwandisa njani amathuba okukhulelwa?

Ngokuchumisa ngokwemvelo, imfihlelo yesigxina sesini ixutywe ngokukodwa kunye neeseli ze-epidermis, ngenxa yoko, utshintsho oluthile kwi-pH yoluntu oluphakathi, olukhokelela ekutshatyalaliswa kwephepha le-lipoprotein le-spermatozoa, i-e. kusebenze. Kuba kule meko, ubomi bee-reproductive cells zithintele kakhulu, ezingabandakanyi ukusetshenziswa kwazo kwi-IVF. Yingakho indlela yokunyusa i- cryopreservation isetyenziswa .

Olu buchule lwandisa kakhulu indawo yokuphila yesithombo sembewu, kwaye ivumela ukuba:

• ukunciphisa umsebenzi we-spermatozoa;
• ubonelela ngolunye umthombo wamandla;
• ukhusela i-membrane yegciwane lesibindi kwintshabalalo;
• kuthintela ukuqokelela kweemveliso ezinobuthi;
• inqanda ukuveliswa kwee-microorganism kwi-ejaculate.

Ziziphi iilwimi ezisetyenziselwa ukugcina isilwanyana?

Ukuze uququzelele, inkqubo ebizwa ngokuba yinto yokuchoboza i-ejaculate, eyimfuneko yokwahlukana kwamaseli egciwane ukusuka kuyo, ngaphambi kokunciphisa isidoda. Iimpawu ezikhethekileyo zisetyenzisiweyo.

Kuza kube yimini, kuyisiko lokusebenzisa imidiya yolungelelaniso yokugcina imbewu, nganye leyo idinga iimeko ezizodwa. Ngokuqhelekileyo, olu hlobo lwendalo luqulethwe ngamalungu amaninzi, ngokuqhelekileyo ubuncinane abathathu. Ngoko, kwisakhiwo sabo kunokwenzeka ukwabelana ngeswekile, ngokuqhelekileyo kwaye kusetyenziswa i-glucose ne-lactose, i-citrate ye-sodium.

Ukuba sithetha ngamakhemikhali athile angasetyenziselwa njengemidiya yokugcina isidoda, ngoko phakathi kwabo kunokubizwa ngokuba yiTris-buffer, Trilon B, EDTA, iSpermosan PPK.

Ngaphansi kweyiphi iimeko i-ejaculate igcinwe?

I-teknoloji yokubanda kunye nokugcina inambuzane idinga ukunamathela kwiprogram epholileyo yokupholisa, kwakunye nokusetyenziswa kwezixhobo ezizodwa. Kule meko, igumbi lebhubhorathi ngokwalo kufuneka lixhotyiswe ngamacebo afaka umoya.

Ngaphambi kokuba i-ejaculate iqokelelwe, zonke iifowuni eziyimfuneko ze-cryopreservation, ngokukodwa zonke iintlobo zeeflasks, izilwanyana eziphunyeziweyo, iipipettes, izihlungi zamaphepha zifakwe kwi-khabhinethi ekhethekileyo, kwiqondo lokushisa lika-130-150 degrees. Ngaphambi kwenkqubo yokutyelela ngokuthe ngqo, zifakwa kwi-thermostat ekhethekileyo, egcina ukufudumala kwama-37 degrees.

Emva kokuba isampula yesilisa isuswe, ifakwe kwisihlunu esingunobumba. Ubushushu bokugcina besidoda kufuneka buhlale bunjalo. Inkqubo yokupholisa iqhutyelwa kwizigaba ezi-2.

Ngowokuqala, i-ejaculate ibeka kuqala kwigumbi elibandayo, apho izinga lokushisa liyehla. Njengomthetho, ixabiso layo li--35 degrees. Emva koko, i-frost deep eyenziwa, iquza ibhola ekhethekileyo kunye nesidoda kwi-nitrogen yamanzi. Kule meko, umphefumlo wesibhedlele unokufikelela kwiminyaka emininzi.

Ukusebenzisa i-ejaculate, i-cryopreserved ngaphambili, inqanawa kunye nayo ifakwe emanzini afudumele, apho i-thawing slowly iqhubeka khona. Emva koko, ama-cryoprotectants asetshenzisiweyo asetshenzisiwe, ngokuphindaphinda ngokuphindaphindiweyo kwesidoda kwi-centrifuge. Emva koko, i-fluinal seminal ngokwayo ithathelwe indawo kunye nomgangatho wesondlo apho i-spermatozoa ifakwa khona.