Okwangoku, ukuhlalutya kwe-PCR kuthathwa njengenye yeendlela ezichanekileyo zokufumanisa izifo ezazisulelayo. Ukongeza, indlela ifikeleleke ngakumbi. Ngenxa yezinga eliphezulu lobuncwane, ukufumana iziphumo ezingamanga kubandakanywa.
Indlela yohlalutyo
Ngethuba lokuhlalutya, izinto zokuvavanya zifakwa kwisixhobo esikhethekileyo. Yongeza i-enzyme echaphazelekayo ekuvelweni kwezinto eziphathekayo. Emva koko kukho ukukopishwa ngokuphindaphindiweyo kwe-DNA okanye i-RNA ye-agent agent. Ukusuka kumjikelo ukuya kumjikelezo, inani leikopi ze-DNA landa kwindleko apho kulula ukuyiqonda i-pathogen.
Ukuhlolwa kwegazi usebenzisa indlela ye-PCR isoloko isetyenziselwa ukwenziwa kweklinikhi ukufumanisa isizathu esibangela isifo esiyingozi. Kwakhona kunokwenzeka ukuba ufunde umchamo, i-smear ukusuka emqaleni kunye nezinye izinto eziphilayo. Kuyabasetyhini, ukuhlaziywa kwe-PCR, izicatshulwa ezivela kwisitho somzimba, isalathisi esivela kwi-urethra , isalathisi yomlomo. Kubalulekile ukwazi indlela yokulungiselela ukuhlalutya kwe-PCR kwabasetyhini, ukwenzela ukuba umphumo unokwethenjelwa kunokwenzeka. Into ephambili ukugcina imithetho elandelayo:
- ukuzilahla kwizenzo zesondo ngaphambi kweentsuku ezintathu ngaphambi kokunikezelwa kweemvavanyo;
- ezininzi iintsholongwane ziyakwazi "ukuhlamba" ngomchamo, ngoko ungagungqi phambi kovavanyo;
- Musa ukwenza uvavanyo emva kwenyanga, kufuneka ulinde iintsuku ezingama-3-5 emva kokuphela.
Ngaphambi kokuhlaziywa kwegazi, akukho malungiselelo okhethekileyo.
I-PCR - uhlalutyo lubonisa ntoni?
Kuyaziwa ukuba uhlalutyo lwe-PCR lubonisa ubukho beentsholongwane ezahlukeneyo zentsholongwane kunye neebhaktheriya. Le ndlela ibuye isebenze ekufumaneni i-latent, infections infections. Uhlalutyo lwe-STI usebenzisa indlela ye-PCR eyenza kube lula ukuhlukanisa i-agent ye-pathogenic nakwii-cells ezilodwa ze-virus kunye neebhaktheriya. Kuyafaneleka ukuba uqaphele ukuba zeziphi iimvavanyo ze-PCR ezifakwe kwiibhloko zokusuleleka koomzimba, ezi zilandelayo:
- Chlamydia;
- mycoplasma ;
- i-ureaplasmas (i-parvum ne-urealiticum);
- i-agentor causative ye-gonorrhea;
- iintlobo ezahlukeneyo zepillomavirus yabantu;
- Trichomonas;
- igadi;
- Abaviwa.
Ngezifo ezithathelwanayo zezitho zangasese, izinto eziphathekayo ze-PCR yi-smear ezivela kwinqanawa yomlomo wesibeleko, i-urethra kunye nesondo. Ukulungiselela ukukhulelwa kufuneka kuhanjiswe ngexanduva elikhulu. Xa uhlela ukukhulelwa, i-PCR ihlalutya imfuneko kwiimeko apho kukho izikhalazo zezifo ezixhaphakileyo. Kwaye ukuba kukho isifo, kungcono ukuyeka ukukhulelwa. Kubalulekile ukuba uqaphele ukuba iimvavanyo zokuchonga izifo ezilapha ngentla kufuneka zingadluliselwa kuphela kumfazi, kodwa nakwindoda.
Kwakhona, indlela ye-PCR ibonisa ezi zifo zilandelayo:
- i-hepatitis B kunye neC virus;
- I-Mycobacterium isifo sofuba;
- HIV;
- iintsholongwane zentsapho ye-herpes, kuquka i-Epstein-Barr negciwane le-cytomegalovirus;
- Usulelo lwe-Helicobacter pylori.
Ukuchazwa kweziphumo
Ukuchongwa kwe-PCR uhlalutyo akubanga iimeko ezinzima. Ngokuqhelekileyo iziphumo zeengcamango ze-PCR zifumaneka ngolu hlobo lulandelayo:
- Iiphumo elibi lichaza ukuba i-arhente efunwayo ayifumanekanga ayifumanekanga kwizinto eziphantsi kokufunda.
- Isiphumo esihle sibonisa ubukho be-DNA okanye i-RNA pathogen. Oko kukuthi, ngokuqinisekileyo kungatsholwa ukuba yi-microorganism echongiweyo yimbangela yesifo.
Kwezinye iimeko, ukuzimisela okuninzi kwamagqabantshintshi kwenziwa. Oku kunjalo ngokukodwa kwizifo ezibangelwa zizilwanyana ezincinci. Ekubeni ezi bhaktheriya zibonisa iimiphumo ezimbi kuphela xa ixabiso ligqithiseleyo. Kwakhona, uhlalutyo lwe-PCR lubalulekile ekukhethweni kweendlela zokunyanga kunye nenjongo yokulawula unyango lwe-HIV kunye ne-virus ye-hepatitis.